SrasV1, Main, Exploration, bibRecord, 003509

Detection of Antibodies Against SARS-Coronavirus Using Recombinant Truncated Nucleocapsid Proteins by ELISA

Identifieur interne : 003509 ( Main/Exploration ); précédent : 003508; suivant : 003510

Detection of Antibodies Against SARS-Coronavirus Using Recombinant Truncated Nucleocapsid Proteins by ELISA

Auteurs : Hyun-Kyoung Lee [Corée du Sud] ; Byoung-Hee Lee [Corée du Sud] ; NOTONKUMAR DUTTA [Corée du Sud] ; Seung-Hyeok Seok [Corée du Sud] ; Min-Won Back [Corée du Sud] ; Hui-Young Lee [Corée du Sud] ; Dong-Jae Kim [Corée du Sud] ; Yi-Rang Na [Corée du Sud] ; Kyoung-Jin Noh [Corée du Sud] ; Sung-Hoon Park [Corée du Sud] ; Hiroaki Kariwa [Japon] ; Mina Nakauchi [Japon] ; LE QUYNH MAI [Viêt Nam] ; Suk-Jin Heo [Corée du Sud] ; Jae-Hak Park [Corée du Sud]

Source :

Journal of microbiology and biotechnology [ 1017-7825 ] ; 2008.

RBID : Pascal:09-0101127

Descripteurs français

KwdFr :
- Anticorps antiviraux (sang), Antigènes viraux (immunologie), Antigènes viraux (isolement et purification), Antigènes viraux (métabolisme), Expression des gènes, Humains, Nucléocapside (immunologie), Nucléocapside (isolement et purification), Nucléocapside (métabolisme), Protéines recombinantes (immunologie), Protéines recombinantes (isolement et purification), Protéines recombinantes (métabolisme), Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (immunologie), Test ELISA (), Virus du SRAS (immunologie).
MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- immunologie : Antigènes viraux, Nucléocapside, Protéines recombinantes, Syndrome respiratoire aigu sévère, Virus du SRAS.
- isolement et purification : Antigènes viraux, Nucléocapside, Protéines recombinantes.
- métabolisme : Antigènes viraux, Nucléocapside, Protéines recombinantes.
- sang : Anticorps antiviraux.
Pascal (Inist)
- Détection, Anticorps, Expression des gènes, Humains, Protéine recombinante, Nucléocapside, Technique ELISA, Coronavirus, Test ELISA.

English descriptors

KwdEn :
- Antibodies, Viral (blood), Antibody, Antigens, Viral (immunology), Antigens, Viral (isolation & purification), Antigens, Viral (metabolism), Coronavirus, Detection, ELISA assay, Enzyme-Linked Immunosorbent Assay (methods), Gene Expression, Humans, Nucleocapsid, Nucleocapsid (immunology), Nucleocapsid (isolation & purification), Nucleocapsid (metabolism), Recombinant Proteins (immunology), Recombinant Proteins (isolation & purification), Recombinant Proteins (metabolism), Recombinant protein, SARS Virus (immunology), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (immunology).
MESH :
- chemical , blood : Antibodies, Viral.
- chemical , immunology : Antigens, Viral, Recombinant Proteins.
- chemical , isolation & purification : Antigens, Viral, Recombinant Proteins.
- chemical , metabolism : Antigens, Viral, Recombinant Proteins.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : Nucleocapsid, SARS Virus, Severe Acute Respiratory Syndrome.
- isolation & purification : Nucleocapsid.
- metabolism : Nucleocapsid.
- methods : Enzyme-Linked Immunosorbent Assay.
- Gene Expression, Humans.

Abstract

Severe acute respiratory syndrome (SARS) is a life-threatening emerging respiratory disease caused by the coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is highly antigenic and may be a suitable candidate for diagnostic applications. We constructed truncated recombinant N proteins (N1 [1-422 aa], N2 [1-109 aa], and N3 [110-422 aa]) and determined their antigenicity by Western blotting using convalescent SARS serum. The recombinants containing N1 and N3 reacted with convalescent SARS serum in Western blotting. However, the recombinant with N2 did not. In ELISA using N1 or N3 as the antigens, positive results were observed in 10 of 10 (100%) SARS-CoV-positive human sera. None of 50 healthy sera gave positive results in either assay. These data indicate that the ELISA using N1 or N3 has high sensitivity and specificity. These results suggest that the middle or C-terminal region of the SARS N protein is important for eliciting antibodies against SARS-CoV during the immune response, and ELISA reactions using N1 or N3 may be a valuable tool for SARS diagnosis.

Affiliations:

Le document en format XML

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<author><name sortKey="Park, Sung Hoon" sort="Park, Sung Hoon" uniqKey="Park S" first="Sung-Hoon" last="Park">Sung-Hoon Park</name>
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<author><name sortKey="Park, Jae Hak" sort="Park, Jae Hak" uniqKey="Park J" first="Jae-Hak" last="Park">Jae-Hak Park</name>
<affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Detection of Antibodies Against SARS-Coronavirus Using Recombinant Truncated Nucleocapsid Proteins by ELISA</title>
<author><name sortKey="Lee, Hyun Kyoung" sort="Lee, Hyun Kyoung" uniqKey="Lee H" first="Hyun-Kyoung" last="Lee">Hyun-Kyoung Lee</name>
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<author><name sortKey="Lee, Byoung Hee" sort="Lee, Byoung Hee" uniqKey="Lee B" first="Byoung-Hee" last="Lee">Byoung-Hee Lee</name>
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<author><name sortKey="Notonkumar Dutta" sort="Notonkumar Dutta" uniqKey="Notonkumar Dutta" last="Notonkumar Dutta">NOTONKUMAR DUTTA</name>
<affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1>
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<author><name sortKey="Seok, Seung Hyeok" sort="Seok, Seung Hyeok" uniqKey="Seok S" first="Seung-Hyeok" last="Seok">Seung-Hyeok Seok</name>
<affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1>
<s2>Seoul 151-742</s2>
<s3>KOR</s3>
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</affiliation>
</author>
<author><name sortKey="Back, Min Won" sort="Back, Min Won" uniqKey="Back M" first="Min-Won" last="Back">Min-Won Back</name>
<affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1>
<s2>Seoul 151-742</s2>
<s3>KOR</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>15 aut.</sZ>
</inist:fA14>
<country>Corée du Sud</country>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lee, Hui Young" sort="Lee, Hui Young" uniqKey="Lee H" first="Hui-Young" last="Lee">Hui-Young Lee</name>
<affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1>
<s2>Seoul 151-742</s2>
<s3>KOR</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
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<sZ>10 aut.</sZ>
<sZ>15 aut.</sZ>
</inist:fA14>
<country>Corée du Sud</country>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kim, Dong Jae" sort="Kim, Dong Jae" uniqKey="Kim D" first="Dong-Jae" last="Kim">Dong-Jae Kim</name>
<affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1>
<s2>Seoul 151-742</s2>
<s3>KOR</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
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<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>15 aut.</sZ>
</inist:fA14>
<country>Corée du Sud</country>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Na, Yi Rang" sort="Na, Yi Rang" uniqKey="Na Y" first="Yi-Rang" last="Na">Yi-Rang Na</name>
<affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1>
<s2>Seoul 151-742</s2>
<s3>KOR</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>15 aut.</sZ>
</inist:fA14>
<country>Corée du Sud</country>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Noh, Kyoung Jin" sort="Noh, Kyoung Jin" uniqKey="Noh K" first="Kyoung-Jin" last="Noh">Kyoung-Jin Noh</name>
<affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1>
<s2>Seoul 151-742</s2>
<s3>KOR</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>15 aut.</sZ>
</inist:fA14>
<country>Corée du Sud</country>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Park, Sung Hoon" sort="Park, Sung Hoon" uniqKey="Park S" first="Sung-Hoon" last="Park">Sung-Hoon Park</name>
<affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1>
<s2>Seoul 151-742</s2>
<s3>KOR</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
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<sZ>8 aut.</sZ>
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<sZ>10 aut.</sZ>
<sZ>15 aut.</sZ>
</inist:fA14>
<country>Corée du Sud</country>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kariwa, Hiroaki" sort="Kariwa, Hiroaki" uniqKey="Kariwa H" first="Hiroaki" last="Kariwa">Hiroaki Kariwa</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University</s1>
<s2>Sapporo 060-0818</s2>
<s3>JPN</s3>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
<country>Japon</country>
<wicri:noRegion>Sapporo 060-0818</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nakauchi, Mina" sort="Nakauchi, Mina" uniqKey="Nakauchi M" first="Mina" last="Nakauchi">Mina Nakauchi</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University</s1>
<s2>Sapporo 060-0818</s2>
<s3>JPN</s3>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
<country>Japon</country>
<wicri:noRegion>Sapporo 060-0818</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Le Quynh Mai" sort="Le Quynh Mai" uniqKey="Le Quynh Mai" last="Le Quynh Mai">LE QUYNH MAI</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Virology Department, National Institute of Hygiene and Epidemiology</s1>
<s2>Yersin Street, Hanoi 10000</s2>
<s3>VNM</s3>
<sZ>13 aut.</sZ>
</inist:fA14>
<country>Viêt Nam</country>
<wicri:noRegion>Yersin Street, Hanoi 10000</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Heo, Suk Jin" sort="Heo, Suk Jin" uniqKey="Heo S" first="Suk-Jin" last="Heo">Suk-Jin Heo</name>
<affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Biological Diagnostic Products Team, Biologics Headquarters, Korea Food and Drug Administration</s1>
<s2>Seoul 122-704</s2>
<s3>KOR</s3>
<sZ>14 aut.</sZ>
</inist:fA14>
<country>Corée du Sud</country>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Park, Jae Hak" sort="Park, Jae Hak" uniqKey="Park J" first="Jae-Hak" last="Park">Jae-Hak Park</name>
<affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1>
<s2>Seoul 151-742</s2>
<s3>KOR</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>15 aut.</sZ>
</inist:fA14>
<country>Corée du Sud</country>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of microbiology and biotechnology</title>
<title level="j" type="abbreviated">J. microbiol. biotechnol.</title>
<idno type="ISSN">1017-7825</idno>
<imprint><date when="2008">2008</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of microbiology and biotechnology</title>
<title level="j" type="abbreviated">J. microbiol. biotechnol.</title>
<idno type="ISSN">1017-7825</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (blood)</term>
<term>Antibody</term>
<term>Antigens, Viral (immunology)</term>
<term>Antigens, Viral (isolation & purification)</term>
<term>Antigens, Viral (metabolism)</term>
<term>Coronavirus</term>
<term>Detection</term>
<term>ELISA assay</term>
<term>Enzyme-Linked Immunosorbent Assay (methods)</term>
<term>Gene Expression</term>
<term>Humans</term>
<term>Nucleocapsid</term>
<term>Nucleocapsid (immunology)</term>
<term>Nucleocapsid (isolation & purification)</term>
<term>Nucleocapsid (metabolism)</term>
<term>Recombinant Proteins (immunology)</term>
<term>Recombinant Proteins (isolation & purification)</term>
<term>Recombinant Proteins (metabolism)</term>
<term>Recombinant protein</term>
<term>SARS Virus (immunology)</term>
<term>Severe Acute Respiratory Syndrome (diagnosis)</term>
<term>Severe Acute Respiratory Syndrome (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (sang)</term>
<term>Antigènes viraux (immunologie)</term>
<term>Antigènes viraux (isolement et purification)</term>
<term>Antigènes viraux (métabolisme)</term>
<term>Expression des gènes</term>
<term>Humains</term>
<term>Nucléocapside (immunologie)</term>
<term>Nucléocapside (isolement et purification)</term>
<term>Nucléocapside (métabolisme)</term>
<term>Protéines recombinantes (immunologie)</term>
<term>Protéines recombinantes (isolement et purification)</term>
<term>Protéines recombinantes (métabolisme)</term>
<term>Syndrome respiratoire aigu sévère (diagnostic)</term>
<term>Syndrome respiratoire aigu sévère (immunologie)</term>
<term>Test ELISA ()</term>
<term>Virus du SRAS (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Viral</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antigens, Viral</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antigens, Viral</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes viraux</term>
<term>Nucléocapside</term>
<term>Protéines recombinantes</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Nucleocapsid</term>
<term>SARS Virus</term>
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Nucleocapsid</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Antigènes viraux</term>
<term>Nucléocapside</term>
<term>Protéines recombinantes</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Nucleocapsid</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antigènes viraux</term>
<term>Nucléocapside</term>
<term>Protéines recombinantes</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Expression</term>
<term>Humans</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Détection</term>
<term>Anticorps</term>
<term>Expression des gènes</term>
<term>Humains</term>
<term>Protéine recombinante</term>
<term>Nucléocapside</term>
<term>Technique ELISA</term>
<term>Coronavirus</term>
<term>Test ELISA</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is a life-threatening emerging respiratory disease caused by the coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is highly antigenic and may be a suitable candidate for diagnostic applications. We constructed truncated recombinant N proteins (N1 [1-422 aa], N2 [1-109 aa], and N3 [110-422 aa]) and determined their antigenicity by Western blotting using convalescent SARS serum. The recombinants containing N1 and N3 reacted with convalescent SARS serum in Western blotting. However, the recombinant with N2 did not. In ELISA using N1 or N3 as the antigens, positive results were observed in 10 of 10 (100%) SARS-CoV-positive human sera. None of 50 healthy sera gave positive results in either assay. These data indicate that the ELISA using N1 or N3 has high sensitivity and specificity. These results suggest that the middle or C-terminal region of the SARS N protein is important for eliciting antibodies against SARS-CoV during the immune response, and ELISA reactions using N1 or N3 may be a valuable tool for SARS diagnosis.</div>
</front>
</TEI>
<affiliations><list><country><li>Corée du Sud</li>
<li>Japon</li>
<li>Viêt Nam</li>
</country>
<region><li>Région capitale de Séoul</li>
</region>
<settlement><li>Séoul</li>
</settlement>
<orgName><li>Université nationale de Séoul</li>
</orgName>
</list>
<tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Lee, Hyun Kyoung" sort="Lee, Hyun Kyoung" uniqKey="Lee H" first="Hyun-Kyoung" last="Lee">Hyun-Kyoung Lee</name>
</region>
<name sortKey="Back, Min Won" sort="Back, Min Won" uniqKey="Back M" first="Min-Won" last="Back">Min-Won Back</name>
<name sortKey="Heo, Suk Jin" sort="Heo, Suk Jin" uniqKey="Heo S" first="Suk-Jin" last="Heo">Suk-Jin Heo</name>
<name sortKey="Kim, Dong Jae" sort="Kim, Dong Jae" uniqKey="Kim D" first="Dong-Jae" last="Kim">Dong-Jae Kim</name>
<name sortKey="Lee, Byoung Hee" sort="Lee, Byoung Hee" uniqKey="Lee B" first="Byoung-Hee" last="Lee">Byoung-Hee Lee</name>
<name sortKey="Lee, Hui Young" sort="Lee, Hui Young" uniqKey="Lee H" first="Hui-Young" last="Lee">Hui-Young Lee</name>
<name sortKey="Na, Yi Rang" sort="Na, Yi Rang" uniqKey="Na Y" first="Yi-Rang" last="Na">Yi-Rang Na</name>
<name sortKey="Noh, Kyoung Jin" sort="Noh, Kyoung Jin" uniqKey="Noh K" first="Kyoung-Jin" last="Noh">Kyoung-Jin Noh</name>
<name sortKey="Notonkumar Dutta" sort="Notonkumar Dutta" uniqKey="Notonkumar Dutta" last="Notonkumar Dutta">NOTONKUMAR DUTTA</name>
<name sortKey="Park, Jae Hak" sort="Park, Jae Hak" uniqKey="Park J" first="Jae-Hak" last="Park">Jae-Hak Park</name>
<name sortKey="Park, Sung Hoon" sort="Park, Sung Hoon" uniqKey="Park S" first="Sung-Hoon" last="Park">Sung-Hoon Park</name>
<name sortKey="Seok, Seung Hyeok" sort="Seok, Seung Hyeok" uniqKey="Seok S" first="Seung-Hyeok" last="Seok">Seung-Hyeok Seok</name>
</country>
<country name="Japon"><noRegion><name sortKey="Kariwa, Hiroaki" sort="Kariwa, Hiroaki" uniqKey="Kariwa H" first="Hiroaki" last="Kariwa">Hiroaki Kariwa</name>
</noRegion>
<name sortKey="Nakauchi, Mina" sort="Nakauchi, Mina" uniqKey="Nakauchi M" first="Mina" last="Nakauchi">Mina Nakauchi</name>
</country>
<country name="Viêt Nam"><noRegion><name sortKey="Le Quynh Mai" sort="Le Quynh Mai" uniqKey="Le Quynh Mai" last="Le Quynh Mai">LE QUYNH MAI</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

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Detection of Antibodies Against SARS-Coronavirus Using Recombinant Truncated Nucleocapsid Proteins by ELISA

Detection of Antibodies Against SARS-Coronavirus Using Recombinant Truncated Nucleocapsid Proteins by ELISA

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